Refresher, Proficiency and Foundation Courses
>> Refresher Courses (30 days)
>> Proficiency Courses
>> Foundation Courses
REFRESHER COURSE (30 days)
In this course emphasis is placed both on understanding the different uses of the advanced instruments as well as the principle behind their functioning. This track provides an excellent training experience for life science students and prepares them for careers in industry, higher education or research.
Fermentation Technology
•Basics of fermenter
•Media preparation & sterilization of the fermenter & usage of various probes involved
•Pre - culture preparation & inoculation
•Observing the growth of the cultures in fermenter
PCR
•DNA isolation & confirmation
•Amplification of the gene of interest
Blotting Techniques
•Southern Blotting - Digesting DNA, Electrophoresis, Transfer of DNA to the membrane & hybridisation
•Western Blotting - SDS PAGE, Transfer of protein to the membrane, Immuno detection
Gel Documentation
•Analysis of gels using gel documentation system
HPLC
•History & principle of HPLC
•Instrumentation & working
•Software & result interpretation
•Chemistry of solvents & solutes
•Analysis of Chromatograms
ELISA
•Diagnosis of one disease – HBsAg / HIV / HCV
•Use of ELISA reader
•Calculation of the cut off values
•Interpretation of the results
Spectrophotometer
Estimation of absorbance, transmittance, absorption maxima, rate of activity, purity and quantification of biomolecules
Bioinformatics
•Retrieving of DNA & protein sequences
•Retrieving protein structures & studying structural features
•Identifying domains & motifs in proteins
•Identification of conserved regions in proteins using a multiple sequence alignment
•Locating transmembrane regions, signal peptides, chloroplast & mitochondrial transit peptides
Plant Biotechnology
•Protoplast Isolation and culturing
•Production of synthetic seeds
•Agrobacterium mediated transformation
PROFICIENCY COURSES
These courses are basically designed to make students proficient in the different techniques used in Biotechnology. The students will be exposed to advanced areas of bioscience which will give them a cutting edge in enhancing the employment opportunity but also the academic advancement. The programme lays more emphasis on hands on training. It gives a thorough practical exposure in handling the most principally used techniques in different avenues of Biotechnology. The modules offered are:
Agricultural Microbiology (20 days)
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Isolation of |
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oCellulose degrading microbes |
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oPectin degrading microbes |
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oStarch degrading microbes |
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oLignin degrading microbes |
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Isolation of rhizobium from root nodules of legumes |
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Isolation of non-symbiotic azotobacter from soil |
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Isolation of associative nitrogen fixer - azospirillum |
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Demonstration of cyanobacterial symbiosis in azolla |
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Demonstration of nitrate reduction by bacteria |
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Assay of biofertilizers |
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Mushroom cultivation |
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Estimation of Protein and carbohydrate content of mushroom |
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Vermi-composting |
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Production of synthetic seeds |
Food Microbiology (20 days)
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Isolation of microbes from fermented foods |
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Study of aflatoxin producing fungi - aspergillus flavus |
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Isolation of microbes from infected fruits and vegetables |
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Isolation of microbes from dairy products |
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Quality analysis of water by MPN test |
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Production of bio - yoghurt |
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Quality analysis of milk |
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Quality analysis of canned food |
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Antimicrobial compounds in food |
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Enumeration of microbes in food by haemocytometer |
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Production and estimation of alcohol from yeast |
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HACCP concepts |
Immunology (20 days)
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Blood collection, separation of serum and plasma |
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Separation of serum proteins on native PAGE |
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Blood smear preparation and visualization of blood Cells |
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Blood grouping |
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Counting of RBCs, WBCs, and platelets |
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Estimation of hemoglobin using haemocytometer |
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Erythrocyte sedimentation rate count |
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Counter current immunoelectroehoresis |
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PCV |
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Widal, RPR, RA, and latex agglutination test |
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Measurement of serum albumin |
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Separation of serum amino acids by paper chromatography |
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RID, RIEP, and ODD |
Genomics (15 days)
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Genomic DNA isolation from bacteria |
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Genomic DNA isolation from plants |
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Genomic DNA isolation from blood |
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Plasmid DNA isolation |
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RNA isolation from yeast |
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Agarose gel electrophoresis |
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Gel elution |
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Restriction digestion |
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Ligation |
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Polymerase chain reaction |
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Phage titration |
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Transformation |
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Softwares and servers for DATA mining |
Proteomics (20 days)
Introduction to Protein Biochemistry
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Structure, classification and importance of proteins |
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Chromatography - basic principles |
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Types of chromatography |
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Introduction to liquid chromatography system |
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Operation of the LC system |
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Purification of industrially important proteins using liquid chromatography |
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Introduction to softwares in proteomics |
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Analysis of the proteins using tools of proteomics |
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Documentation of experiments |
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Report preparation |
Plant Biotechnology (20 days)
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Stock solution and chemical preparation |
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Media preparation |
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In vitro culturing technique |
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oPrimary Culture - Seed, embryo, nodal, internodal, leaf, storage organ, nucellus, anther, shoot tip, root
tip, suspension culture |
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oSub Culture - Callus, shoot induction, root induction, embryo induction |
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Production of synthetic seeds |
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Agrobacterium mediated transformation |
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Protoplast isolation |
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Genomic DNA isolation from plant sample |
Clinical Biochemistry (15 days)
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Qualitative analysis of Urine |
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Estimation of blood Glucose |
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Estimation of Serum Proteins |
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Estimation of serum amino acids |
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Estimation of serum Albumin - Globulin ratio |
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Estimation of Serum SGOT, SGPT, LDH |
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Estimation of DNA |
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Estimation of RNA |
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Estimation of Uric acid |
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Estimation of Cholesterol. |
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Lipid Profile test |
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Thin layer chromatography |
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Estimation of free fatty acids |
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Thin Layer Chromatography |
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Estimation of Serum Amino cids |
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Assay of Enzymes for the effect of pH, Temperature, Enzyme Concentration, Substrate Concentration, |
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Activators, Inhibitors |
Industrial Biotechnology (20 days)
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Isolation, Screening and Mass production of industrially important micro organisms
Enzyme producing microorganism
Antibiotic producing microorganism
Microorganisms used as biofertilizers |
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Immobilization of Enzymes/cells |
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Wine Production and Estimation of alcohol content in the produced wine |
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Study of Growth curve of microorganisms |
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Study of fermenter operations |
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Precipitation |
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Dialysis |
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Chromatography |
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oThin layer chromatography |
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oIon exchange chromatography |
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oGel filtration chromatography |
Chromatographic (10 days)
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Paper Chromatography |
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Thin Layer Chromatography |
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Ion exchange Chromatography |
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Gel Filtration Chromatography |
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Affinity Chromatography |
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HPLC Analysis of pharmaceutical formulations |
FOUNDATION COURSES
Foundation courses are short term introduction courses offered in different departments and disciplines in Biotechnology. They aim at baptizing the student towards Biotechnology and providing them with the basic platform for more advanced research. The training modules compromise individual hands-on training and serve as an introductory exposure to learning highly advanced modules in future.
You could choose from the following modules:
Department of Industrial Biotechnology
•Immobilization of Cells
•Isolation & screening for industrially important bacteria
•Antibiotic production & testing its efficacy
•Aflatoxin production
•Thin Layer chromatography
•Quality analysis of milk
Department of Immunology
•Separation of serum & plasma from blood
•Erythrocyte Sedimentation Rate (ESR)
•Blood Grouping
•Blood smear preparation, Staining & visualization of blood cells
•Blood counts:
oCounting of WBC
oRBC
oPlatelets
•Estimation of Blood Haemoglobin using Haemometer
•ELISA – Tridot
•Widal, Latex agglutination tests
•Measurement of serum albumin
•Separation of serum amino acids by paper chromatography
•RID
Department of Molecular Biology
•Orientation and instrumentation
•Calculations and Chemical preparation
•Isolation of Plasmid DNA
•Isolation of Genomic DNA from Bacteria
•Total RNA Isolation from Yeast
•Agarose Gel Electrophoresis
•Conjugation
Department of Recombinant DNA Technology
•Orientation and instrumentation
•Calculations and Chemical preparation
•Isolation of Plasmid DNA
•Isolation of Genomic DNA from Bacteria
•Isolation of total RNA from Yeast.
•PCR Amplification of DNA
•Agarose Gel Electrophoresis
•Restriction Digestion
•Ligation
Department of Plant Biotechnology
•Stock solution and Chemical Preparation
•Media Preparation
•Invitro regeneration techniques
oOrganogenesis - Nodal culture & leaf culture
oSomaticembryogenesis - Nuspension culture
oCallogenesis - Callus culture
oRhizogenesis - Invitro rooting
•Estimation of plant secondary metabolites
•Estimation of chlorophyll
•Production of artificial seeds
•Plant Genomic DNA Isolation
•Agarose Gel Electrophoresis
Department of Clinical Biochemistry
•Qualitative analysis of urine samples
•Estimation of glucose from blood and urine
•Level of iron and hemoglobin in blood
•Lipid profile Test - Estimation of cholesterol, Estimation HDL, Estimation LDL, Estimation Triglycerides,
Estimation of Lactate dehydrogenase
•Liver function tests - Determination of SGOT and SGPT activity, Estimation of bilirubin, Determination of •Albumin/Globulin ratio in serum
Department of Bioinformatics
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Bioinformatics – History and development and future challenges. Basics of Linux operating system. |
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The nucleotide sequence databases. Genome databases. Obtaining information from nucleotide and protein sequence databases. Other types of biological databases.
Software/Servers PubMed,NCBI,EMBL,SwissProt.,KEGG. |
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Sequence comparison by alignment. Types of alignment – global and local. Scoring an alignment – substitution matrices and gap penalties. BLAST and its applications.
Software/Servers : Lalign, BLAST |
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Multiple sequence alignment and Phylogenetic analysis.
Software/Servers : Clustal X, ,Phylip. |
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Nucleotide sequence analysis – Gene prediction, primer design for PCR. Restriction digestion,
Software/Servers :EMBOSS, GENSCAN, ORFFinder, Primer3,NEBWebcutte.r |
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Protein sequence analysis – studying physico-chemical characteristics, locating signal and transit peptides. Identifying motifs and domains.
Software/Servers : ExPaSy, PROSITE, Pfam, |
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Introduction to protein structure – Protein structure databases.
Software/Servers : PDB, MMDB,CATH,SCOP. |
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Visualizing protein structure data
Software/Tools-Rasmol |
