Capsule Courses
>> HPLC - Advanced Module (6 days)
>> Liquid Chromatography (4 days)
HPLC (3 days)
Basic Module
History and Principle
Instrumentation and working
Software Usage
Result Interpretation
HPLC - Advanced Module (6 days)
History and principle
Instrumentation and working
Software usage
Solvent preparation
Sample injection
Result interpretation
Estimation of agrochemicals/pharmaceutical compounds using a preset method
ELISA (4 days)
Enzyme-linked immunosorbent assay (ELISA) is a useful and powerful method in estimating ng/ml to pg/ml ordered materials in the solution, such as serum, urine and culture supernatant. Students possessing the skill to work with ELISA would always have a cutting edge in the field of clinical biology.
Module:
Diagnosis of Diseases like HIV/HBsAg/HCV/ etc using ELISA Reader and washer
The student is familiarized with ELISA Reader and ELISA Washer
Calculation of the cut off values
Interpretation of the results
Rapid diagnosis of different disease using Tri Dot ELISA, an immuno chromatographic techniques (ICT)
PCR: Basic Module (3 days)
Isolation of genomic DNA
Agarose gel electrophoresis
Specific gene amplification and confirmation
PCR: Advanced Module (12 days)
Specific gene amplification
Colony PCR
Gradient PCR
RAPD PCR
Multiplex PCR
Blotting techniques (5 days)
Southern Blotting
Western Blotting
Gene Transfer Techniques (6 days)
Conjugation
Phage Titration
Transformation
Operation of Fermenter (5 days)
Basics of fermenter
Media preparation & Insitu sterilization of the fermenter
Pre - culture preparation
Inoculation
Observing the growth of the cultures in fermenter
Usage of various probes involved
Screening of Transgenic Plants (3 days)
Isolation of plant genomic DNA
Agarose gel electrophoresis
Specific gene amplification and confirmation of transgene
DNA Fingerprinting (6 days)
Random Amplification of Polymorphic DNA
Restriction Fragment Length Polymorphism
ISSR
